Preliminary Programme 22nd ERGECD Amsterdam
Thursday, September 24th
20.30 – 22.00 Welcome with Tapas and Drinks: WTCafé de Blauwe Engel (WTC Amsterdam, Strawinskylaan 143, 1077 XX Amsterdam)
Friday, September 25
08.00 - 08.30 a.m. Registration and Welcome (VU medical center, De Boelelaan 1117, 1081HV Amsterdam. Main Hospital Entrance, Lecture Theater ‘Maaszaal’)
Session 1 Allergen reactivity profiling
* Please note: 10 minutes for each presentation (including high priority questions). Discussion and summing up at the end of session).
08.30 - 10.00 a.m.
Functional Proteomics of the Skin
Erika Parkinson, Paul Skipp, Maja Aleksic, Andrew Garrow, Geraldine Clough, Daniel Scott and David O’Connor
Proteomic Isolation and Mass Spectrometric Identification of DNBS Specific Target Proteins and Peptides in Human Skin
Lisa Dietz, Irina Goette, Stefanie Ohnesorge, Stefanie Eikelmeier, Martina Schnölzer, Herman-Josef Thierse
Targeting the skin barrier: proteomic studies on nickel-protein interactions in human keratinocytes.
Stefanie Ohnesorge, Lisa Dietz, Patrick Pankert, Hermann-Josef Thierse
Progress in peptide reactivity profiling
Maja Aleksic, Emma Thain, Delphine Roger, Ouarda Saib, Michael Davies, Jin Li, Nora Aptula and Raniero Zazzeroni
Exploring the Immunologic Synapse in Allergic Contact Dermatitis by Modelling the T-Cell Receptor/Major Histocompatibility-/Peptide-Complex
Stefan R. Ahlfors, Anders Sundin, Christer Hansson
Chemical behaviour of mixtures of sensitizers towards nucleophilic peptides
Matthieu Lang, Elena Giménez-Arnau, Jean-Pierre Lepoittevin
Fragrance contact allergy: chemical reactivity of terpenic allylic hydroperoxides toward amino acids in iron systems
Dany Kao, Elena Giménez-Arnau, Alain Chaintreau, Jean-Pierre Lepoittevin
Clustering of Corticoid molecules and delayed hypersensibility reactions
JA. Chemelle, M Baeck, A. Goossens, J.F. Nicolas, R. Terreux
Adduct formation of Limonene Hydroperoxides
Anna Börje
Potential Cause of Photocontact Allergy to Sunscreens: Photodegradation of Dibenzoylmethanes
Isabella Karlsson
Coffee break 10.10-10.40 a.m.
10.40-11.20 a.m.
Metabolism of Diphenylthiourea
Kristin Samuelsson, Moa Andresen Bergström, Charlotte Jonsson, Gunnar Westman, Lars G. Nilsson, Ann-Therese Karlberg
A Structure-Activity Relationship Study on Epoxides as Contact Allergens
Ida Belogorcev Niklasson
Oxidized linalool: a common sensitizer – hydroperoxides identified in perfumes
Johanna Nilsson, Johanna Bråred-Christensson, Ann-Therese Karlberg
Mechanistic chemistry insights in the development of intelligent risk assessment strategies for skin sensitisation
Nora Aptula, Bob Safford, Nicky Gilmour. (Paper 1)
Dermal Sensitisation Threshold – Integrating mechanistic chemistry insights
Bob Safford, Nora Aptula and Nicky Gilmour. (Paper 1)
11.30 - 12.00 Discussion and Summing up Session 1 Chair: Jean Pierre Lepoittevin
Lunch break 12.00 - 13.00
Session 2 DC / T/ Treg / NK cells, immune reactivity
13.00 - 14.40 p.m.
The role of epidermal signals in the induction of skin sensitisation
Vicki Summerfield
Accumumulation of FITC near stratum corneum – visualizing epidermal distribution of a strong sensitizer using two-photon microscopy
Kristin Samuelsson, Carl Simonsson, Charlotte Jonsson, Gunnar Westman, Marica Ericson, Ann-Therese Karlberg
The stratum corneum contains a biochemical redox barrier which protects against sensitisation by highly reactive environmental chemicals
C Pickard, F Louafi, K Lowings, R Dearman, I Kimber, E Healy, PS Friedmann
In vivo metabolism of Hyaluronic acid triggered by contact allergens
Philipp Eßer, Verena Weber, Stefan martin
Phenotypic changes in migrating Langerhans cells after irritant exposure
K. Ouwehand, T.D. de Gruijl, R.J. Scheper, S. Gibbs
Implication of ROS and detoxification pathways in dendritic cells activation by chemical sensitizers
Philippe Rousseau , Nadège Ade , Diane Antonios , Saadia Kerdine-Römer and Marc Pallardy
Investigation of the xenobiotic metabolising competency of dendritic cells
Dawn Yates, Steve Clifton, Ruth Pendlington & Camilla Pease
Role of oxidative stress, calcium and MAPKs in dendritic cell activation induced by contact sensitizers
C. Migdal1, L. Foggia1, A. Ferrer1, M. Tailhardat2, P. Courtellemont2, M. Haftek1, M. Serres1
Histamine Receptor H1 expression on Dendritic Cells Regulates Atopic Dermatitis.
Béatrice Vanbervliet, Mubeccel Akdis, Marc Vocanson Aurore Rozières, Karen Rodet, Jean-François Nicolas and Ana Hennino
Role of IL-2 in cutaneous immune responses to 2,4-dinitrochlorobenzene
Rebecca Dearman, Craig Portsmouth, Christopher Griffiths and Ian Kimber
Tea break 14.40 - 15.20 p.m.
15.20 - 17.00 p.m.
DNTB: basis for unique cutaneous immune responses
Ian Kimber, Marie Cumberbatch, Craig Portsmouth and Rebecca Dearman
Skin immune tolerance to haptens: the example of dinitrothiocyanobenzene.
Marc Vocanson, Gaelle Poyet, Josette Bénetières, Jean-François Nicolas
Innate immune mechanisms in chemical-induced contact dermatitis – Toll like receptors and more
Martin S
Natural Killer cell involvement in Contact Hypersensitivity.
Paul Rouzaire, Carmelo Luci, Elisabeth Blasco, Jacques Bienvenu, Eric Vivier, Jean-François Nicolas, Elena Tomasello and Ana Hennino
Prevention of Diabetes in NOD Mice by Exposure to a Contact Allergen
Engkilde, K
Oral Exposure to metal allergens
Muris, Joris
In vivo and in vitro studies on metal allergy
Hoogstraten, Ingrid van
Increased frequency of metal allergy in patients with multiple sclerosis compared with healthy controls
Stejskal, Vera
Skin Sensitization to p-phenylenediamine: The role of exposure time and dose for the elicitation response in allergic patients
Carsten Goebel, Pieter-Jan Coenraads, Monika Kock, Brunhilde Blömeke
Detection of specific T cells in drug allergy : diagnosis application.
Aurore Rozieres, Jerome Valois, Marc Vocanson, Paul Rouzaire, Jacques Bienvenu, Ana Hennino and Jean-Francois nicolas
17.00 - 17.30 Discussion and Summing up Session 2 Chair: Rik Scheper
Canal Boat Ride and Drinks 18.30 - 20.00
Buffet Dinner 20.00 - 22.30
Saturday, September 26
Session 3 Allergenicity biosensors
8.30 - 10.10 a.m.
Gene profiling of the human keratinocyte cell line HaCaT discriminates contact sensitizers from irritants
Rob Vandebriel, Jeroen Pennings, Kirsten Baken, Andre Boorsma, Ralph Gottschalk & Henk van Loveren
Keratinocyte-THP-1 Cultures - A New Approach for Studying the Sensitizing Effects of Small Chemicals
Brunhilde Blömeke, Jennifer Hennen, Jutta Bonifas, Judith Clemens, Michaela Kalmes, Axel Meyer, Carsten Goebel, Pierre Aeby, Klaus Fischer
Development of a new high-throughput keratinocyte-based standard assay to detect skin sensitizers based on ARE-dependent gene activity: Development of an SOP and validation vs. standard lists of reference chemicals
Natsch, A, Emter, R. and Ellis, G.
Evaluation of HMOX1 and IL-8 as complementary biomarkers in a THP-1 sensitization test based on h-CLAT protocol
Hadi S., Peiffer J-L., Bonnet P-A., Fabre I., Ourlin j-c.
Analysis of physico-chemical properties of test chemicals for considering of the applicability domain of cell line-based skin sensitization test (h-CLAT)
Ashikaga, Takao
Prediction of skin sensitization potential of chemicals in human by human Cell Line Activation Test (h-CLAT) and an attempt of classification of skin sensitization potency with the test
Nukada, Yuko
Case study – Interpretation of new data
Trannum Rahim, Maja Aleksic, Nora Aptula, Nicola Gilmour, Bob Safford
Potential epidermal cell and dendritic cell-based tests for assessing sensitization.
J. Reinders, Sens-it-iv consortium, S. Gibbs
Mitogen-activated protein kinases control Nrf2 accumulation in human dendritic cells in response to the chemical sensitizers.
Authors: Saadia Kerdine-Römer, Diane Antonios, Philippe Rousseau and Marc Pallardy
A closer look at novel skin sensitization markers in dendritic cells
N. Lambrechts, H. Vanheel, J. Hooyberghs, R. Van Den Heuvel, H. Witters, I. Nelissen, G. Schoeters
Assessment of chemical skin sensitizing potency by an in vitro assay based on human dendritic cells
N. Lambrechts1, J. Hooyberghs1, H. Vanheel1, H. Witters1, R. Van Den Heuvel1, V. Van Tendeloo2, I. Nelissen1, G.Schoeters
Coffee break 10.20 - 10.50 a.m.
10.50 - 12.40 a.m.
The Applicability Domain of the LLNA
Steven J Enoch, David W Roberts, Fania Bajot, Mark TD Cronin
Assessment of the sensitising potential of textile disperse dyes and some of their metabolites by Loose-fit Coculture-based Sensitisation Assay (LCSA)
Sonnenburg, A, Wanner, R
Combined in-vitro classification of sensitizing and irritative potential by the LCSA
Reinhard Wanner, Anna Sonnenburg
The T cell priming assay predicts the allergenicity of chemicals: current status and perspectives.
Amine Achachi, Marc Vocanson, Gaelle Poyet, Cyril Chavagnac, Magalie Valeyrie, Jean-François Nicolas
Epidermal disposition kinetics of sensitising chemicals for the purposes of risk assessment.
Authors: Michael Davies, Ruth Pendlington and Cameron MacKay
Mechanism-based modelling of skin and respiratory sensitization
David W Roberts, Steven J Enoch, Mark TD Cronin
Development of an elicitation model to assess if potency ranking of contact sensitizers is comparable with LLNA data
Janine Ezendam, Arja de Klerk, Rob Vandebriel, Henk van Loveren
Integration of non-animal data for hazard assessment of skin sensitisers
Cameron MacKay
Comparing test results from different regulatory sensitization tests
Nicholas Ball, Stuart Cagen, Juan-Carlos Carrillo, Hans Certa, Dorothea Eigler, Christine Garcia, Harald Esch, Cynthia Graham, Carl Haux, Reinhard Kreiling and Annette Mehling
Updating the skin sensitisation in vitro data assessment paradigm
David Basketter, Ian Kimber
12.40 - 13.00 Discussion and Summing up Session 3 Chair: Sue Gibbs
Meeting closure 13.00 hours
Lunch 13.00 - 14.00
